Next, a minigene encompassing the region around ADARB1 exon 4 and 5 was cloned into pDESTsplice splicing reporter plasmid and PCR mutagenesis was used to introduce the c.1245_1247+1 del variant (figure 3H).13 This construct was then transiently transfected into SH-SY5Y (metastatic neuroblastoma) and HeLa (cervical cancer) cells. This evidence concerns the gene ADARB1 and cervical cancer.