To model the effects of hyperinsulinemia in vitro and characterize the effects of prolonged insulin exposure in adipocytes, we incubated mouse 3T3-L1 and human SGBS to a supraphysiological dose of insulin (150 nM), which was added to the medium every day from day 6 post differentiation induction until differentiation to mature adipocytes. Here, INS is linked to hyperinsulinism.