To investigate the consequences of UPF3A frameshift mutations on the proteomic landscape of CRC cells, UPF3A-proficient (+dox, pUPF3A), and UPF3A-deficient (dox, dUPF3A), cells were compared by analyzing global protein expression as well as associated phosphorylation changes. This evidence concerns the gene UPF3A and colorectal carcinoma.