Furthermore, the mechanism that underlies the beneficial effect of the TRF paradigm on obesity is still not welldefined.Various mechanisms have been proposed to explain the anti-obesity effect of a TRF regimen, includingentraining the circadian clock to a fixed feeding time [32], altering gut microbiome [33], and increasing free fatty acid mobilization and fat oxidation [8]. This evidence concerns the gene CLOCK and obesity due to melanocortin 4 receptor deficiency.