(3)The association between more severe amputations (outcome 3, with disarticulation and bilateral) at older age (adjusted PR = 1.55, 95% CI: 1.12–2.14), male gender (adjusted PR = 1.83, 95% CI: 1.27–2.64), alcoholism (adjusted PR = 1.70, 95% CI: 1.25–2.33), insulin use (adjusted PR = 0.68, 95% CI: 0.48–0.97). The gene discussed is INS; the disease is alcohol drinking.