As such, we identified DEGs such as MYC, miRNAs such as miR-125a-3p, miR-940, miR-375 miR-196a, miR-21, miR-205, miR-194, miR-103, miR-223, and miR-198a, and pathways such as the proteasome, p53, and beta-alanine metabolism pathways, which may be involved in ESCC development. This evidence concerns the gene TP53 and esophageal squamous cell carcinoma.