Since development of this approach it has been widely used, e.g., for highly efficient PCSK9-targeted genome editing via zinc-finger nuclease (ZFN) mRNAs, that were delivered to the liver by lipid nanoparticles (63); and to demonstrate the gender-dependent role of the EC-mineralocorticoid receptor (MR) for atherosclerosis and vascular inflammation, revealing the potential of EC-MR inhibitor therapy in male patients (64). This evidence concerns the gene PCSK9 and atherosclerosis.