Elevated Rho GTPase activity has been reported to play a key role in the hyper‐proliferative and invasive behaviors of cancer cells.[27, 28] Thus we evaluated the ability of DC‐Rhoin04 to inhibit cellular Rho activity and Rho‐mediated cell migration and invasion in MDA‐MD‐231 cells, a highly aggressive triple‐negative breast cancer cells.[29] The cellular activity of RhoA was assessed by quantification of the amount of GTP‐loaded RhoA from cellular lysates using RBD pull down assay. This evidence concerns the gene RHOA and cancer.