In order to demonstrate whether NF-κB was the core factor modified by altered methylation induced by sepsis, we verified 4 more signaling pathways, including the MAPK signaling pathway, Hippo signaling pathway, EGFR signaling pathway, and the ErbB signaling pathway, which were placed at the top in the KEGG of differentially expressed genes and methylation variable positions, (Supplementary Figure 4; Figure 1C). The gene discussed is NFKB1; the disease is Sepsis.