In the present study, the methylation-based markers for CRC included LGR6, PTPN12, PPFIA3, LOC399959, PCDHGA1, RNF39, ESYT3, MRGPRG and ATHL1, all of which were located in the promoter regions or first introns of nearby genes. The gene discussed is PPFIA3; the disease is colorectal carcinoma.