It means that TSA had a more significant effect on the up-regulation of p21Cip1/Waf1/Sdi1, p27Kip1, p57Kip2. In consistent with our result, it has been reported that zebularine can restore p21Cip1/Waf1/Sdi1 in colon cancer SW48 and HT-29 (Flis et al., 2014), CT15 and HT-29 colon cancer (Cheng et al., 2004). This evidence concerns the gene CDKN1A and malignant colon neoplasm.