To determine whether pyroptosis occurred in TAC hearts, and whether it was closely related to MLK3, the SEM results showed more inflammasomes and membrane rupture in TAC + AAVNC mice, membrane were completely ruptured in the stage of late pyroptosis in the improved pictures, while more apoptotic cells were found in TUNEL staining in TAC + AAVNC mice compared with those in AAVMLK3− mice (Fig. 3j, k). This evidence concerns the gene MAP3K11 and persistent truncus arteriosus.