HIF1A and colorectal carcinoma: Moreover, colony formation assay, soft agar assay, healing assay, transwell assay and three-dimensional morphogenesis assay showed that overexpression of OSBPL3 could antagonize the effect of HIF1A interference on the progression of CRC in vitro (Fig. 5e–h and Supplementary Fig. S6A-E), which further light on the relation of OSBPL3 with the regulation of HIF1A.