GSEA bioinformatics analysis revealed that many biological processes and pathways were potentially involved in the OSBPL3-promoted CRC progression, the results showed that these genes with co-expression characteristics of OSBPL3 were mainly involved in cell focal adhesion, cytoskeleton regulation and other tumor-related signaling pathways (Supplementary Fig. S4A and Fig. 4a). Here, OSBPL3 is linked to colorectal carcinoma.