To further verify the regulatory effect of HIF1A on OSBPL3, we treated CRC cells with CoCl2 (simulated hypoxia).The results showed that, compared with the control group, the expression levels of OSBPL3 in CRC cell lines RKO and HCT15 were significantly increased with different concentrations of CoCl2, showing a concentration-dependent relationship (Fig. 5a). The gene discussed is OSBPL3; the disease is colorectal carcinoma.