To validate the involvement of ATP7A in KRAS-dependent tumor growth, we performed soft agar assays using KRAS IEC-6 (Supplementary Fig. 4f) and KRAS-mutated CRC (Supplementary Fig. 4g) cells, and found that shRNA-mediated ATP7A knockdown reduced anchorage-independent growth. This evidence concerns the gene KRAS and colorectal carcinoma.