The aim of our prospective observational study was to evaluate the influence of the tissue-type plasminogen activator (r-tPA) dose level in patients treated with MTE for acute ischemic stroke (AIS) related to an occlusion of middle cerebral artery (MCA) by comparing outcomes and complication rates among the patients treated by bridging therapy (BT groups) or direct MTE alone (dMTE group). This evidence concerns the gene PLAT and androgen insensitivity syndrome.