Using IFNγ-ELISpot (enzyme-linked immunospot) assay, spontaneous CD4+ and CD8+ T cells specific for sBARF1 protein were identified in the peripheral blood from both EBV-seropositive donors and NPC patients but not in EBV-seronegative controls [55]. The gene discussed is CD8A; the disease is nasopharyngeal carcinoma.