qRT-PCR results showed the CYLD knockout induces upregulation of a panel of NF-kB downstream genes (Figure S7); we examined the expression of these CYLD-targeted genes in the single-cell data and discovered that there are almost twice as many cells with hyperactivated NF-kB activity in the cancer cells with the CYLD knockout compared to the control (Figure 5A). Here, NFKB1 is linked to cancer.