MIOX and obesity due to melanocortin 4 receptor deficiency: In addition, we crossbred MIOX-KO (MIOXKO) with leptin-deficient ob/ob mice to generate an ob/MIOXKO in order to assess if the disruption of MIOX confers a rescuing effect in states of obesity with respect to tubulo-interstitial injury reflected in the levels of tubular injury biomarkers, i.e., KIM-1 and NGAL (Figure 1).