ESR1 and neoplasm: In contrast, a study by Minemura et al. reported that 20% of breast carcinoma cases are positive for miR-1, whereas non-neoplastic mammary glands did not express miR-1 as determined by in situ hybridization (ISH), and that miR-1 expression is associated with ER status, PR status, tumor stage, tumor grade, and distant and LN metastasis [87].