The mRNA expression of transporters CHT1, CTL1-5, and OCT1,2, which are active transporters of choline in normal human mammary epithelial cell line MCF-10A and human pancreatic-cancer cell lines MIA PaCa-2 and PANC-1, was detected by qPCR (Figure 1A). This evidence concerns the gene SLC5A7 and familial pancreatic carcinoma.