They demonstrated that inhibition of tumor acidosis by adenylyl cyclase inhibitor MDL-12 induces NOS, CXCL9, CXCL11 and TNF-α-expressing proinflammatory TAMs that phenotypically resemble classical M1 macrophages, leading to suppression of tumor growth in B16 mouse melanoma [63]. This evidence concerns the gene NOS2 and melanoma.