Moreover, it has been mechanistically confirmed that PGRMC1 directly interacts and regulates EGFR levels in lung and breast cancer cells.18 Similar studies have demonstrated that this interaction regulates tumour proliferation mainly through the phosphorylation of EGFR, AKT and ERK in colon cancer cells.10 Interestingly, silencing PGRMC1 in lung and breast cancer cells was found to decrease the expression of total EGFR.18 In the present study, GO analysis results suggest that PGRMC1 exerts its effects mainly through PI3K/AKT signalling. This evidence concerns the gene PGRMC1 and breast cancer.