Next, colony formation assays were carried out to evaluate the anti-proliferative effects of alpelisib and/or paclitaxel on five GC cell lines (Fig. 2C): two PIK3CA wild-type (SNU638 and SNU668) and three PIK3CA-mutant cells (SNU601, AGS, and MKN1). This evidence concerns the gene PIK3CA and gastric cancer.