AIF1 and hydrops fetalis: To study HF-induced microglial changes in the PVN and CeA, we assessed microglial surface area, cell volume, filament length, microglial branches, microglial segments, filament terminals, and IBA1 intensity in sham and HF rats at 8, 14, and 16 weeks after the surgery (n = 4/group) (Fig. 1).