Herein, our research revealed downregulation of miR-141-3p in NEC, and receptor-interacting protein kinase 1 (RIPK1) functioned as the direct downstream molecule of miR-141-3p to aggravate intestinal epithelial cell injury by activating receptor-interacting protein kinase 3 (RIPK3)/mixed lineage kinase domain like pseudokinase (MLKL)-mediated necroptotic signaling pathway, which might contribute to the pathogenesis of NEC. Here, MLKL is linked to necrotizing enterocolitis.