In the current study, silencing of FASN, FSCN1 or SPTBN1 expression in liver cancer cells led to changes in the mRNA expression of EMT-associated markers, E-cadherin, N-cadherin, vimentin and transcription factors Snail and Twist, and altered the protein expression of MMP-2 and MMP-9. Here, TWIST1 is linked to liver cancer.