In order to examine the effects of prebiotics on the T cell populations including Th1, Th2, Th17, and Treg, we analyzed the mRNA expression of their specific transcription factors and cytokines such as T-bet, GATA3, RORγt, Foxp3, interferon (IFN)-γ, interleukin(IL)-4, IL-17, and TGF-β, respectively, in the mesenteric lymph nodes (MLN) derived from the DNCB-induced AD mouse model. Here, TGFB1 is linked to Alzheimer disease.