The prebiotic (T1~T3) and Zyrtec (P) groups clearly showed an increase in mRNA expression of Th1 and Treg-specific transcription factors and cytokines such as T- bet, IFN-γ, Foxp3, and TGF-β compared with the DNCB-induced AD control (N) group (Figs. 2A and 2D). Here, TGFB1 is linked to Alzheimer disease.