To understand the potential functional impact of ‘M1hot TAMs’ on antitumor immunity, we classified our cohort of cancer subjects based on the expression of M1 marker genes such as CXCL9, CXCL10, CXCL11, CXCL12, STAT1, FAM26F (heatmap, figure 4A), choosing CXCL9 as the candidate gene to classify tumors into M1hot (top 25th percentile) M1intermediate (25th–75th percentile) and M1cold (bottom 25th percentile) tumors (figure 4A). The gene discussed is CXCL12; the disease is cancer.