SOCS3 and cancer: CXCL9 is a chemokine previously known as monokine induced by IFN-γ, overexpressed by macrophages after stimulation by IFN-γ (the key cytokine behind M1 signature activation) and is involved in recruitment of antitumor T cells as well as having anti-angiogenic roles and has been suggested as a novel target for cancer therapy.35 We thus correlated the expression of CXCL9, as one of the strongest M1 marker genes in macrophages, with key M2 marker genes (CD209, ADORA3, STAT6, SOCS3, IL10 and IRF4) (figure 2D, online supplementary figure S2).