In the present study, there was no major difference in IL-7 production between NKG2D-CAR and NKG2DIL7-CAR T cells in the absence of tumor, but when co-cultured with PC-3 cells, IL-7 expression in NKG2DIL7-CAR-transduced T cells increased vigorously. The gene discussed is KLRK1; the disease is neoplasm.