When a metastatic tissue sample is not available, the analyses may be performed on samples obtained from lymph node metastases or primary tumor since a high concordance of the BRAF status between primary melanomas and their metastatic lesions has been demonstrated (Colombino et al., 2012; Casula et al., 2016; Valachis and Ullenhag, 2017; Cormican et al., 2019; Pellegrini et al., 2020). This evidence concerns the gene BRAF and melanoma.