We evaluated the status of femur BM vasculature during leukemia progression by immunofluorescence staining of blood vessel walls with intravenously injected Alexa-Fluor-647-conjugated VE-cadherin antibody (37) and in calvaria BM by intravital 2-photon imaging of dextran-TRITC-labeled blood flow. The gene discussed is CDH5; the disease is leukemia.