Given the significant correlation between high expression of KIF20A and poor prognosis, we further analyzed the mRNA and protein expression of KIF20A among five CRC cell lines by RT-PCR and Western blot analysis, respectively (Figures 3(a) and 3(b)), and found that HCT116 and SW1116 had high expression of KIF20A, so they were chosen for subsequent study. Here, KIF20A is linked to colorectal carcinoma.