Both subpopulations of effector T lymphocytes, CD4+T helper cells (p < 0.001, Figure 4(a)) and CD8+CTLs (p < 0.001, Figure 4(d)), were significantly reduced in the melanomas of B16F10+MSC14d-treated mice compared to B16F10+PBS14d-treated animals. This evidence concerns the gene CD4 and melanoma.