The results (Fig. 6a) demonstrated that FOS, HGF, IGF2, FOXO1, and NTF3 were remarkably downregulated while IGF1R expression was notably elevated in ICC tissues (|logFC| > 2, FDR < 0.01) compared to that of adjacent normal tissues. This evidence concerns the gene FOXO1 and intrahepatic cholangiocarcinoma.