Following the release of CCL2 by tumour cells and TAMs, cytotoxic T lymphocytes acquire CD4/CD25 expression and become immunosuppressive regulators (Treg cells).92 By producing TGF-β and IL-10, Treg cells contribute to an immunosuppressive environment through the inhibition of cytotoxic T cells and NK cells. This evidence concerns the gene TGFB1 and neoplasm.