Consistent with previous reports, LVS tagged with a fluorescent reporter (GFP-LVS or mCherry-LVS) was phagocytosed by macrophages and began to escape from EEA1+ or LAMP1+ phagosomes within 1 to 2 h of infection (Supplementary Fig. 1, illustrating partial LVS colocalization with LAMP1 by 4 h after infection). The gene discussed is EEA1; the disease is infection.