HMGCR and infection: To compare the effects of inhibiting sphingolipid metabolism to inhibiting other lipid metabolic pathways known to play a role in the flavivirus replication cycle38, we treated Huh7 cells with lovastatin (inhibitor of HMGCR, a key enzyme in cholesterol biosynthesis) or PIK93 (inhibitor of phosphatidylinositol-4-kinase) before infection with ZIKV; in contrast to myriocin and FB1, lovastatin treatment caused a more modest 30% decrease in viral shedding by 24 hpi, and PIK93 did not appear to decrease infection (Supplementary Fig. 6a, b).