Indeed, depletion of either PKCα or WWP1 abrogated TRIB3-enhanced tumor sphere formation (Fig. 5i); while TRIB3 depletion-impaired tumor sphere formation could be rescued by overexpressing the of EGFRT654D mutant but not the EGFRT654A or EGFRT654D/K689R mutants (Supplementary Fig. 4g). Here, TRIB3 is linked to neoplasm.