To determine the mechanism underlying SPINK1 action in Enz-resistant PCa, we generated stable SPINK1-knockdown (shSPINK1) C4-2R and C4-2B-R cells, and SPINK1-overexpressing (oeSPINK1) C4-2 and C4-2B cells (Figure 2A). This evidence concerns the gene SPINK1 and posterior cortical atrophy.