Our study revealed that low PIP-TMZ treatment resulted in increased phosphorylation of JNK and p38 MAPK in U251-MG and T98G cells, implying that low concentration PIP-TMZ combination could induce cell death through the JNK/p38 MAPK-mediated intrinsic apoptotic pathway in GBM cells. The gene discussed is MAPK8; the disease is glioblastoma.