S100A8 and infection: Using CRISPR/Cas9 gene knockout, Zhao et al. [1] unexpectedly reported that neither heterodimers of integrin-β1 nor integrin-αv are essential for translocation of CagA by a type IV secretion system (T4SS) and for induction of the cell elongation phenotype by H. pylori. We performed infection experiments using the reported AGS wild-type and isogenic knockout cells for integrin-β1 (ΔITGB1) and integrin-αv/integrin-β4 (ΔITGAvB4) [1] with various worldwide T4SS-positive strains including TN2-GF4, G27 and P12 used by the authors.