Furthermore, mitochondrial membrane potential was significantly reduced (Figure 4D, 4E) and ROS levels were significantly higher (Figure 4F, 4G) in the selisistat-pretreated Mst1-knockdown RA-FLSs compared to the control Mst1-knockdown RA-FLSs upon H2O2 treatment. This evidence concerns the gene MST1 and rheumatoid arthritis.