IGF1 and perinatal asphyxia: To address this issue, a study investigating the impact of temporal hypoxia-ischemia on IGF-1 secretion by particular subpopulations of CNS glia was designed, based on the in vitro (glial primary cell monocultures), ex vivo (hippocampal organotypic slice culture), and in vivo (rat model of perinatal asphyxia) experiments (Fig. 1).