However, among ISV + α-CTLA-4 treated mice we observed significantly increased CD8+ T cell infiltration in the extracranial LF tumor compared with the intracranial brain tumor (p<0.05 by ANOVA with post hoc Bonferroni; for ISV + α-CTLA-4 treated mice - LF: 230±13 per 20X field, BrMet: 130±16; for untreated mice - LF: 49±11, BrMet: 36±13; mean±SE, n≥5) (figure 4C). Here, CD8A is linked to neoplasm.