However, no significant differences were observed in tumor infiltration by FOXP3+ T cells, though a non-significant trend was observed toward an increase in tumors from ISV + α-CTLA-4 treated mice (for ISV + α-CTLA-4 - LF: 40±14 per 20X field, BrMet: 61±21; for untreated - LF: 5.8±0.28, BrMet: 8.2±2.5; mean±SE, n≥5) (figure 4B). This evidence concerns the gene FOXP3 and neoplasm.