CD40 and neoplasm: Agonistic CD40 antibody has previously been suggested to synergize with αPD1 checkpoint blockade by increasing DC-mediated T-cell priming or by blocking PDL1 upregulation.34 35 First, we measured the expression of CD40 on APCs in pre-treatment MC38 tumors, tumor-draining lymph nodes, distal lymph nodes and spleens.