Furthermore, to assess the biological functions of MK5 in cell proliferation, migration and invasion of CRC, we knocked down or overexpressed MK5 in HCT116 and SW620 cells by transfecting with si-MK5 (si-MK5 #1, siMK5 #2, siMK5 #3) or with an overexpression plasmid (pcDNA3.1-MK5) (Fig. S3B). Here, MAPKAPK5 is linked to colorectal carcinoma.