To assess the biological functions of MK5-AS1 in cell proliferation, migration and invasion of CRC, transient transfection of MK5-AS1-expressing cell lines was performed, wherein pcDNA3.1 MK5-AS1 and siMK5-AS1-siRNA (si-MK5-AS1 #1, si-MK5-AS1 #2, si-MK5-AS1 #3, si-MK5-AS1 #4) were used for overexpression and knockdown functional studies, respectively. The gene discussed is MAPKAPK5; the disease is colorectal carcinoma.