We established a variety of tumor cells, i.e., OVCAR-3 cells, OVCAR3-PDL1-luc cell, OVCAR3-MUC16-GFP-luc cell, and OVCAR3-PDL1-MUC16-GFP-luc cells to better simulate antigenic expression in OC patients. This evidence concerns the gene MUC16 and neoplasm.