In order to determine the efficacy of CAR-T cells against ovarian cancer cells in vivo, we established intraperitoneal tumor-bearing models using NPG mice, which were injected with OVCAR3-MUC16-GFP-PDL1-luc cells (5 × 105 cells) and 50 μL Matrigel into the abdominal cavity and raised 48 h. Here, MUC16 is linked to ovarian cancer.