Finally, ELISA was carried out to evaluate the effects of hyperoside on the production of proinflammation factors in periodontitis rats, and the data of Fig. 1E demonstrated that the production of TNF‐α, IL‐1β and IL‐6 was overexpressed in periodontitis rats, whereas hyperoside treatment could notably inhibit the production of TNF‐α, IL‐1β and IL‐6. This evidence concerns the gene IL1B and periodontitis.