To identify high-confidence chaperone binding partners of human HSC70 and HSP70, we used human osteosarcoma (U2OS) cell lines containing stably integrated, doxycycline-inducible HSC70-UBAIT (wild-type and ΔGG) and HSP70-UBAIT (wild-type and ΔGG) chaperones and grew these cells for 3 days after chaperone induction. This evidence concerns the gene HSPA1A and osteosarcoma.