To elucidate the potential mechanisms underlying the roles of RP11-295G20.2 in HCC cells, we firstly conducted the nuclear/cytoplasmic fractionation assay to determine the subcellular location of RP11-295G20.2 and found that RP11-295G20.2 was primarily expressed in the cytoplasm of HCC cells (Figure 3A). Here, CYDAER is linked to hepatocellular carcinoma.